Development of continuous cultivation of CHO cells producing recombinant blood coagulation factor VIII
Abstract
Perfusion cultivation process for CHO cells producing recombinant B-domen deleted coagulation factor VIII was developed. Cell culture medium and feed supplement were optimized by screening of big panel of prototype media and supplements. Cultivation temperature was optimized for better expression of complex protein as well. Cultivation process was tested in a laboratory-scale perfusion bioreactor. Significant increase of productivity was shown as a result of the development.
About the Authors
A. N. Morozov
International Biotechnological Center «Generium»
Russian Federation
Russian Federation
G. D. Sidelnikov
International Biotechnological Center «Generium»
Russian Federation
Russian Federation
I. M. Emelyanov
International Biotechnological Center «Generium»
Russian Federation
Russian Federation
K. E. Lapshin
International Biotechnological Center «Generium»
Russian Federation
Russian Federation
D. R. Alimova
International Biotechnological Center «Generium»
Russian Federation
Russian Federation
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Review
For citations:
Morozov A.N., Sidelnikov G.D., Emelyanov I.M., Lapshin K.E., Alimova D.R. Development of continuous cultivation of CHO cells producing recombinant blood coagulation factor VIII. BIOpreparations. Prevention, Diagnosis, Treatment. 2015;(4):26-31. (In Russ.)
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