Determination of thimerosal in non-adsorbed immunobiological preparations by cold vapor atomic absorption spectroscopy (CV AAS) for the ions of mercury. Part 1: method development and evaluation of statistical significance of differences in the results of mercury ions determination by colorimetric method and cold vapor atomic absorption spectroscopy method
Abstract
The article describes development of methods of quantitative determination of thimerosal in non-adsorbed immunobiological preparations by cold vapor atomic absorption spectroscopy as well as an estimation of the statistical significance of differences in the results determination of thimerosal conventional colorimetric method in combination with dithizone and CV AAS using one-way analysis of variance with Fisher’s exact test (F-test).
About the Authors
O. N. Kolesnikova
Federal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
O. B. Ustinnikova
Federal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
O. B. Runova
Federal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
V. P. Bondarev
Federal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
References
1. Иммунизация, вакцины и биологические препараты. Тиомерсал. ВОЗ; 2011.
2. Волкова Р.А., Гавриленкова В.Ю., Каргина T.M., Штанчаева С.М., Конду Э.И., Эльберт Е.В., Рунова В.Ф., Блоха В.В., Черняховская И.В. Физикохимические, химические, физические и иммунохимические методы контроля медицинских иммунобиологических препаратов. Фармакопейная статья. ФС 42-3874-99. М.; 1999.
3. British Pharmacopoeia 2013, Mercury A84, Atomic Absorption Spectrometry A171.
4. European Pharmacopoeia. 7th edition, 2.2.23. Atomic Absorption Spectrometry.
5. Thiomersal in vaccines. Extract from report of GACVS meeting 6-7 June 2012. Published WHO Weekly Epidemiological Record, 27 July 2012.
6. Material Safety Data Sheet. Thiomersal MSDS. Joint Commission on Accreditation and Healthcare Organization (JCAHO)
7. Perkin Elmer Corporation, 2012. WinLab32 for AA program. User Manual.
8. Veda Dzeti. J. Chem. Soc. Japan, Pure Chem Sect. 1971; 92: 418.
9. Wolf G.K. Kerntechnik, 1965; 7: 122; C.A., 66, 81337c (196)
10. Hirai M., Hagatsu R. 1 Pharmac Soc Japan 1950; 70: 670.
11. Ldutard J., Pateau L. Chim analyt. 1956; 38: 158.
12. Lopez-Escobar L., Hume D.N. Analyt. Letters 1973; 6: 343. References
Review
For citations:
Kolesnikova O.N., Ustinnikova O.B., Runova O.B., Bondarev V.P. Determination of thimerosal in non-adsorbed immunobiological preparations by cold vapor atomic absorption spectroscopy (CV AAS) for the ions of mercury. Part 1: method development and evaluation of statistical significance of differences in the results of mercury ions determination by colorimetric method and cold vapor atomic absorption spectroscopy method. BIOpreparations. Prevention, Diagnosis, Treatment. 2015;(2):59-63. (In Russ.)
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