Kit for diagnosis of BK virus infection by the real-time Polymerase Chain Reaction method (PCR)
Abstract
The presented work comprises information about developed by Republic Scientific and Practical Center for Epidemiology and Microbiology the diagnostic kit for detection the BK virus DNA in clinical samples by the real-time Polymerase Chain Reaction method. Accomplished studies showed high level its diagnostic sensitivity and specificity. Suitability of developed kit for laboratory BK virus diagnosis was demonstrated.
About the Authors
K. L. Dziadziulia
ГУ «Республиканский научно-исследовательский центр эпидемиологии и микробиологии»
Russian Federation
Russian Federation
T. V. Amvrosieva
ГУ «Республиканский научно-исследовательский центр эпидемиологии и микробиологии»
Russian Federation
Russian Federation
N. V. Paklonskaya
ГУ «Республиканский научно-исследовательский центр эпидемиологии и микробиологии»
Russian Federation
Russian Federation
Z. F. Bogush
ГУ «Республиканский научно-исследовательский центр эпидемиологии и микробиологии»
Russian Federation
Russian Federation
V. A. Zemlianski
ГУ «Республиканский научно-исследовательский центр эпидемиологии и микробиологии»
Russian Federation
Russian Federation
S. K. Loziuk
ГУ «Республиканский научно-исследовательский центр эпидемиологии и микробиологии»
Russian Federation
Russian Federation
References
1. Eash S., Manley K., Gasparovic M., Querbes W., Atwood W.J. The human polyomaviruses. Cell Mol Life Sci. 2006; 63(7-8): 865-76.
2. Bennett S.M., Broekema N.M., Imperiale M.J. BK polyomavirus: emerging pathogen. Microbes Infect. 2012; 14(9): 672-83.
3. De Gascun C.F., Carr M.J. Human polyomavirus reactivation: disease pathogenesis and treatment approaches. Clin Dev Immunol. 2013; 2013: 373579.
4. Jiang M., Abend J.R., Johnson S.F., Imperiale M.J. The role of polyomaviruses in human disease. Virology. 2009; 384(2): 266-73.
5. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol Biol Evolution 2013; 30: 2725-9.
6. Bárcena-Panero A., Echevarria J.E., Romero-Gómez M.P., Royuela E., Castellanos A., González I., Fedele G. Development and validation with clinical samples of internally controlled multiplex real-time PCR for diagnosis of BKV and JCV infection in associated pathologies. Comp Immunol Microbiol Infect Dis. 2012; 35(2):173-9.
Review
For citations:
Dziadziulia K.L., Amvrosieva T.V., Paklonskaya N.V., Bogush Z.F., Zemlianski V.A., Loziuk S.K. Kit for diagnosis of BK virus infection by the real-time Polymerase Chain Reaction method (PCR). BIOpreparations. Prevention, Diagnosis, Treatment. 2015;(3):58-60. (In Russ.)
Views:
1086
Email this article 