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Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library

Abstract

The construction of highly sensitive new-generation diagnostic test systems requires the use of modern innovative technologies that are capable of detecting infectious agents at the molecular level by identifying specific antibodies to individual immunoreactive epitopes. The development of such immunoassays is based on molecular mapping of a target antigen using a library of short overlapping peptides aimed at identification of both epitopes specific for a particular pathogen, and those common to the group of infectious agents. The article describes new approaches that are based on the results of mapping of the Omptin group model protein that could be critical for improving retrospective laboratory diagnosis of infectious diseases caused by bacteria of the Enterobacteriaceae family. The article discusses the prospects for assessing the applicability of the peptide ELISA experimental version, designated as p6/24-Omptin-TIFA, for the detection of antibodies to diagnostically significant Omptin epitopes in the serum of people who have had enterobacterial infections, in order to introduce it into healthcare practices.

About the Authors

V. A. Feodorova
Federal Research Center for Virology and Microbiology (FRCVM) - Branch in Saratov; N. I. Vavilov Saratov State Agrarian University
Russian Federation
Chief research associate of the Laboratory of Molecular Biology and Nanobiotechnologies. Doctor of Medical Sciences, professor


M. A. Khizhnyakova
Federal Research Center for Virology and Microbiology (FRCVM) - Branch in Saratov; N. I. Vavilov Saratov State Agrarian University
Russian Federation
Junior research associate of the Laboratory of Molecular Biology and Nanobiotechnologies


S. S. Zaitsev
Federal Research Center for Virology and Microbiology (FRCVM) - Branch in Saratov
Russian Federation
Research associate of the Laboratory of Molecular Biology and Nanobiotechnologies


A. M. Lyapina
Federal Research Center for Virology and Microbiology (FRCVM) - Branch in Saratov
Russian Federation
Research associate of the Laboratory of Molecular Biology and Nanobiotechnologies


L. V. Sayapina
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation
Chief expert. Doctor of Medical Sciences


E. P. Lyapina
Saratov State Medical University named after N. I. Razumovsky
Russian Federation
Professor of the Department of Infectious Diseases. Doctor of Medical Sciences


O. V. Ulyanova
Federal Research Center for Virology and Microbiology (FRCVM) - Branch in Saratov
Russian Federation
Senior research associate of the Laboratory of Molecular Biology and Nanobiotechnologies. Candidate of Medical Sciences, assistant professor


V. L. Motin
University of Texas Medical Branch
Russian Federation
Professor of the Department of Pathological Conditions and the Department of Microbiology and Immunology. Candidate of Biological Sciences


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Review

For citations:


Feodorova V.A., Khizhnyakova M.A., Zaitsev S.S., Lyapina A.M., Sayapina L.V., Lyapina E.P., Ulyanova O.V., Motin V.L. Evaluation of diagnostic potential of immunoreactive epitopes of the Omptin protease family by using a peptide library. BIOpreparations. Prevention, Diagnosis, Treatment. 2017;17(3):180-186. (In Russ.)

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ISSN 2221-996X (Print)
ISSN 2619-1156 (Online)