Preview

БИОпрепараты. Профилактика, диагностика, лечение

Расширенный поиск

Новые антирабические рекомбинантные вакцины

Полный текст:

Аннотация

Обзор посвящен проблемам получения новых антирабических вакцин с помощью рекомбинантных технологий. Новые подходы к созданию антирабических вакцин включают методы обратной генетики, получение антигенов вируса бешенства в культурах растительных клеток, получение вирусоподобных частиц и конструирование ДНК-вакцин и вакцин на основе различных вирусных векторов. Методы обратной генетики позволяют с помощью плазмид конструировать аттенуированные штаммы вируса бешенства. Накопление основного антигена вируса бешенства - гликопротеина G в культурах растительных клеток является перспективным с точки зрения получения «съедобных» вакцин, не требующих тщательной очистки антигена и многократного парентерального введения. Вирусоподобные частицы способны нести сразу несколько антигенов вируса бешенства, а также различные молекулярные адъюванты. ДНК-вакцины характеризуются простотой получения и невысокой стоимостью, однако требуют различных способов повышения иммуногенности. Большой интерес представляют кандидатные антирабические вакцины на основе различных вирусных векторов, экспрессирующих ген основного антигена вируса бешенства - гликопротеина G. На сегодняшний момент активно применяют ветеринарные вакцины на основе рекомбинантных вируса осповакцины и аденовируса человека пятого серотипа. Репликативно-деффектный аденовирус человека пятого серотипа является перспективным кандидатом и при создании вакцин для массовой иммунизации населения.

Об авторах

Е. С. Седова
Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени почетного академика Н. Ф. Гамалеи
Россия
Научный сотрудник лаборатории молекулярной биотехнологии, канд. биол. наук


М. М. Шмаров
Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени почетного академика Н. Ф. Гамалеи
Россия
Руководитель лаборатории молекулярной биотехнологии, д-р биол. наук


Список литературы

1. Davis BM, Rall GF, Schnell MJ. Everything you always wanted to know about rabies virus (but were afraid to ask). Annu Rev Virol. 2015; 2(1): 451-71.

2. World Health Organization. WHO Expert Consultation on Rabies. Second Report. World Health Organ Tech Rep Ser. 2013; (982): 1-139.

3. Willoughby RE Jr. Rabies: rare human infection - common questions. Infect Dis Clin North Am. 2015; 29(4): 637-50.

4. Эпидемиологический надзор. О ситуации по бешенству в Российской Федерации [Интернет] 2015 [cited 2016 Sept 12] Available from: http://rospotrebnadzor.ru/deyatelnost/epidemiological-surveillance /?ELEMENT ID=5610&sphrase id=731393.

5. Стародубова ЕС, Преображенская ОВ, Кузьменко ЮВ, Латанова АА, Ярыгина ЕИ, Карпов ВЛ. Вакцины против бешенства: современное состояние и перспективы развития. Молекулярная биология 2015; 49(4): 577-84.

6. Kaur M, Garg R, Singh S, Bhatnagar R. Rabies vaccines: where do we stand, where are we heading? Expert Rev Vaccines. 2015; 14(3): 369-81.

7. Rupprecht CE, Nagarajan T, Ertl H. Current status and development of vaccines and other biologics for human rabies prevention. Expert Rev Vaccines. 2016; 15(6):731-49.

8. Hicks DJ, Fooks AR, Johnson N. Developments in rabies vaccines. Clin Exp Immunol. 2012; 169(3):199-204.

9. Conzelmann KK, Schnell M. Rescue of synthetic genome RNA analogs of rabies virus by plasmid encoded proteins. J Virol. 1994; 68(2):713-9.

10. Blanton JD, Self J, Niezgoda M, Faber ML, Dietzschold B, Rupprecht C. Oral vaccination of raccoons (Procyon lotor) with genetically modified rabies virus vaccines. Vaccine 2007; 25(42): 7296-300.

11. Tao L, Ge J, Wang X, Wen Z, Zhai H, Hua T, et al. Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production. Virol J. 2011; 8: 454.

12. Liu X, Yang Y, Sun Z, Chen J, Ai J, Dun C, et al. A recombinant rabies virus encoding two copies of the glycoprotein gene confers protection in dogs against a virulent challenge. PLoS One 2014; 9(2): e87105.

13. Сафонов ГА, Баньковский ДО. Оценка антигенных и иммунологических свойств штамма ERA G333 вируса бешенства. Вестник российской сельскохозяйственной науки 2010; (5): 61-3.

14. Yang DK, Nakagawa K, Ito N, Kim HH, Hyun BH, Nah JJ, et al. A single immunization with recombinant rabies virus (ERAG3G) confers complete protection against rabies in mice. Clin Exp Vaccine Res. 2014; 3(2): 176-84.

15. Shuai L, Feng N, Wang X, Ge J, Wen Z, Chen W, et al. Genetically modified rabies virus ERA strain is safe and induces long-lasting protective immune response in dogs after oral vaccination. Antiviral Res. 2015; 121: 9-15.

16. Cenna J, Hunter M, Tan GS, Papaneri AB, Ribka EP, Schnell MJ, et al. Replication-deficient rabies virus-based vaccines are safe and immunogenic in mice and nonhuman primates. J Infect Dis. 2009; 200(8): 1251-60.

17. Zhao L, Toriumi H, Wang H, Kuang Y, Guo X, Morimoto K, et al. Expression of MIP-1alpha (CCL3) by a recombinant rabies virus enhances its immunogenicity by inducing innate immunity and recruiting dendritic cells and B cells. J Virol. 2010; 84(18): 9642-8.

18. Wen Y, Wang H, Wu H, Yang F, Tripp RA, Hogan RJ, et al. Rabies virus expressing dendritic cell-activating molecules enhances the innate and adaptive immune response to vaccination. J Virol. 2011; 85(4): 1634-44.

19. Barkhouse DA, Garcia SA, Bongiorno EK, Lebrun A, Faber M, Hooper DC. Expression of interferon gamma by a recombinant rabies virus strongly attenuates the pathogenicity of the virus via induction of type I interferon. J Virol. 2015; 89(1): 312-22.

20. Norton JE, Lytle AG, Shen S, Tzvetkov EP, Dorfmeier CL, McGettigan JP. ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo. PLoS One 2014; 9(1): e87098.

21. Rosales-Mendoza S. Current developments and future prospects for plant-made biopharmaceuticals against rabies. Mol Biotechnol. 2015; 57(10): 869-79.

22. McGarvey PB, Hammond J, Dienelt MM, Hooper DC, Fu ZF, Dietzschold B, et al. Expression of the rabies virus glycoprotein in transgenic tomatoes. Biotechnology 1995; 13(13): 1484-7.

23. Ashraf S, Singh PK, Yadav DK, Shahnawaz M, Mishra S, Sawant SV, et al. High level expression of surface glycoprotein of rabies virus in tobacco leaves and its immunoprotective activity in mice. J Biotechnol. 2005; 119(1): 1-14.

24. Loza-Rubio E, Rojas-Anaya E, López J, Olivera-Flores MT, Gómez-Lim M, Tapia-Pérez G. Induction of a protective immune response to rabies virus in sheep after oral immunization with transgenic maize, expressing the rabies virus glycoprotein. Vaccine 2012; 30(37): 5551-6.

25. Rojas-Anaya E, Loza-Rubio E, Olivera-Flores MT, Gómez-Lim M. Expression of rabies virus G protein in carrots (Daucus carota). Transgenic Res. 2009; 18(6): 911-9.

26. Roy S, Tyagi A, Tiwari S, Singh A, Sawant SV, Singh PK, et al. Rabies glycoprotein fused with B subunit of cholera toxin expressed in tobacco plants folds into biologically active pentameric protein. Protein Expr Purif. 2010; 70(2): 184-90.

27. Skarjinskaia M, Ruby K, Araujo A, Taylor K, Gopalasamy-Raju V, Musiychuk K, et al. Hairy roots as a vaccine production and delivery system. Adv Biochem Eng Biotechnol. 2013; 134: 115-34.

28. Singh A, Srivastava S, Chouksey A, Panwar BS, Verma PC, Roy S, et al. Expression of rabies glycoprotein and ricin toxin B chain (RGP-RTB) fusion protein in tomato hairy roots: A step towards oral vaccination for rabies. Mol Biotechnol. 2015; 57(4): 359-70.

29. Perea Arango I, Loza Rubio E, Rojas Anaya E, Olivera Flores T, Gonzalez de la Vara L, Gómez Lim MA. Expression of the rabies virus nucleoprotein in plants at high levels and evaluation of immune responses in mice. Plant Cell Reports 2008; 27(4): 677-85.

30. Modelska A, Dietzschold B, Sleysh N, Fu ZF, Steplewski K, Hooper DC, et al. Immunization against rabies with plant-derived antigen. Proc Natl Acad Sci USA. 1998; 95(5): 2481-5.

31. Yusibov V, Hooper DC, Spitsin SV, Fleysh N, Kean RB, Mikheeva T, et al. Expression in plants and immunogenicity of plant virus-based experimental rabies vaccine. Vaccine 2002; 20(25-26): 3155-64.

32. Lua LHL, Connors NK, Sainsbury F, Chuan YP, Wibowo N, Middelberg APJ. Bioengineering virus-like particles as vaccines. Biotechnol Bioeng. 2014; 111(3): 425-40.

33. Hua RH, Li YN, Chen ZS, Liu LK, Huo H, Wang XL, et al. Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese encephalitis virus for a subunit vaccine candidate. BMC Biotechnol. 2014; 14: 62.

34. Fontana D, Kratje R, Etcheverrigaray M, Prieto C. Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate. Vaccine 2015; 33(35): 4238-46.

35. Kang H, Qi Y, Wang H, Zheng X, Gao Y, Li N, et al. Virus-like particles containing membrane-anchored GM-CSF enhances the immune response against rabies virus. Viruses 2015; 7(3): 1134-52.

36. Qi Y, Kang H, Zheng X, Wang H, Gao Y, Yang S, et al. Incorporation of membrane-anchored flagellin or Escherichia coli heat-labile enterotoxin B subunit enhances the immunogenicity of rabies virus-like particles in mice and dogs. Front Microbiol. 2015; 6: 169.

37. Abdulhaqq SA, Weiner DB. DNA vaccines: developing new strategies to enhance immune responses. Immunol Res. 2008; 42(1-3): 219-32.

38. Xiang ZQ, Spitalnik S, Tran M, Wunner WH, Cheng J, Ertl HC. Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. Virology 1994; 199(1): 132-40.

39. Perrin P, Jacob Y, Aguilar-Sétien A, Loza-Rubio E, Jallet C, Desmézičres E, et al. Immunization of dogs with a DNA vaccine induces protection against rabies virus. Vaccine 1999; 18(5-6): 479-86.

40. Lodmell DL, Ray NB, Parnell MJ, Ewalt LC, Hanlon CA, Shaddock JH, et al. DNA immunization protects nonhuman primates against rabies virus. Nat Med. 1998; 4(8): 949-52.

41. Margalith M, Vilalta A. Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine. Genet Vaccines Ther. 2006; 4: 2.

42. Lodmell DL, Ray NB, Ulrich JT, Ewalt LC. DNA vaccination of mice against rabies virus: effects of the route of vaccination and the adjuvant monophosphoryl lipid A (MPL). Vaccine 2000; 18(11-12): 1059-66.

43. Pinto AR, Reyes-Sandoval A, Ertl HCJ. Chemokines and TRANCE as genetic adjuvants for a DNA vaccine to rabies virus. Cell Immunol. 2003; 224(2): 106.

44. Lodmell DL, Parnell MJ, Bailey JR, Ewalt LC, Hanlon CA. Rabies DNA vaccination of non-human primates: post-exposure studies using gene gun methodology that accelerates induction of neutralizing antibody and enhances neutralizing antibody titers. Vaccine 2002; 20(17-18): 2221-8.

45. Borhani K, Ajorloo M, Bamdad T, Mozhgani SH, Ghaderi M, Gholami AR. A comparative approach between heterologous prime-boost vaccination strategy and DNA vaccinations for rabies. Arch Iran Med. 2015; 18(4): 223-7.

46. Bahloul C, Taieb D, Diouani MF, Ahmed SB, Chtourou Y, B’Chir BI, et al. Field trials of a very potent rabies DNA vaccine which induced long lasting virus neutralizing antibodies and protection in dogs in experimental conditions. Vaccine 2006; 24(8): 1063-72.

47. Стародубова ЕС, Кузьменко ЮВ, Латанова АА, Преображенская ОВ, Карпов ВЛ. Создание ДНК-вакцинного вектора на основе кодон-оптимизированного гена гликопротеина (белка G) вируса бешенства с консенсусной аминокислотной последовательностью. Молекулярная биология 2016; 50(2): 376-80.

48. Kaur M, Rai A, Bhatnagar R. Rabies DNA vaccine: no impact of MHC class I and class II targeting sequences on immune response and protection against lethal challenge. Vaccine 2009; 27(15): 2128-37.

49. Kaur M, Saxena A, Rai A, Bhatnagar R. Rabies DNA vaccine encoding lysosome-targeted glycoprotein supplemented with Emulsigen-D confers complete protection in preexposure and postexposure studies in BALB/c mice. FASEB J. 2010; 24(1): 173-83.

50. Kramps T, Probst J. Messenger RNA-based vaccines: progress, challenges, applications. Wiley interdisciplinary reviews: RNA 2013; 4(6): 737-49.

51. Schnee M, Vogel AB, Voss D, Petsch B, Baumhof P, Kramps T, et al. An mRNA vaccine encoding rabies virus glycoprotein induces protection against lethal infection in mice and correlates of protection in adult and newborn pigs. PLoS Negl Trop Dis. 2016; 10(6): e0004746.

52. Clinicaltrials.gov RNActive®Rabies vaccine (CV7201) in Healthy Adults [Internet] 2016 [cited 2016 August 12] Available from: https://clinicaltrials.gov/ct2/show/NCT02241135?term=rabies+vaccine&rank=39.

53. Draper SJ, Heeney JL. Viruses as vaccine vectors for infectious diseases and cancer. Nat Rev Microbiol. 2010; 8(1): 62-73.

54. Седова ЕС, Щербинин ДН, Мигунов АИ, Смирнов ЮА, Логунов ДЮ, Шмаров ММ и др. Гриппозные рекомбинантные вакцины. Acta naturae 2012; 4(15): 17-27.

55. Wiktor TJ, Macfarlan RI, Reagan KJ, Dietzschold B, Curtis PJ, Wunner WH, et al. Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene. Proc Natl Acad Sci USA. 1984; 81(22): 7194-8.

56. Follmann E, Ritter D, Swor R, Dunbar M, Hueffer K. Preliminary evaluation of Raboral V-RG® oral rabies vaccine in Arctic foxes (Vulpes lagopus). J Wildl Dis. 2011; 47(4): 1032-5.

57. Amann R, Rohde J, Wulle U, Conlee D, Raue R, Martinon O, et al. A new rabies vaccine based on a recombinant ORF virus (parapoxvirus) expressing the rabies virus glycoprotein. J Virol. 2013; 87(3): 1618-30.

58. Marrow JC, Padilla LR, Hayek LA, Bush M, Murray S. Comparison of antibody response to a nonadjuvanted, live canarypox-vectored recombinant rabies vaccine and a killed, adjuvanted rabies vaccine in eld’s deer (Rucervus eldi thamin). J Zoo Wildl Med. 2014; 45(2): 315-20.

59. Li Z, Wang J, Yuan D, Wang S, Sun J, Yi B, et al. A recombinant canine distemper virus expressing a modified rabies virus glycoprotein induces immune responses in mice. Virus Genes 2015; 50(3): 434-41.

60. Yuan Z, Zhang S, Liu Y, Zhang F, Fooks AR, Li Q, et al. A recombinant pseudorabies virus expressing rabies virus glycoprotein: Safety and immunogenicity in dogs. Vaccine 2008; 26(10): 1314-21.

61. Huang Y, Chen Z, Huang J, Fu Z, He B. Parainfluenza virus 5 expressing the G protein of rabies virus protects mice after rabies virus infection. J Virol. 2015; 89(6): 3427-9.

62. Astray RM, Ventini DC, Boldorini VL, Silva FG, Rocca MP, Pereira CA. Rabies virus glycoprotein and immune response pattern usingrecombinant protein or recombinant RNA viral vectors. Vaccine 2014; 32(24): 2829-32.

63. Wu Q, Yu F, Xu J, Li Y, Chen H, Xiao S, et al. Rabies-virus-glycoprotein-pseudotyped recombinant baculovirus vaccine confers complete protection against lethal rabies virus challenge in a mouse model. Vet Microbiol. 2014; 171(1-2): 93-101.

64. Карпов АП, Тутыхина ИЛ, Логунов ДЮ, Верховская ЛВ, Шмаров ММ, Валихов АФ и др. Конструирование рекомбинантных аденовирусов птиц CELO, экспрессирующих гены гликопротеинов gB, gE, gI вируса болезни Марека. Биотехнология 2007; (5): 38-44.

65. Тутыхина ИЛ, Щербинин ДН, Шмаров ММ, Логунов ДЮ, Народицкий БС. Преимущества и перспективы использования генетических вакцин для защиты от опасных и социально значимых инфекций. Вестник РАМН 2011; (10): 37-49.

66. Zhang S, Liu Y, Fooks AR, Zhang F, Hu R. Oral vaccination of dogs (Canis familiaris) with baits containing the recombinant rabies-canine adenovirus type-2 vaccine confers long-lasting immunity against rabies. Vaccine 2008; 26(3): 345-50.

67. Hu RL, Liu Y, Zhang SF, Zhang F, Fooks AR. Experimental immunization of cats with a recombinant rabies-canine adenovirus vaccine elicits a long-lasting neutralizing antibody response against rabies. Vaccine 2007; 25(29): 5301-7.

68. Zhao J, Liu Y, Zhang S, Fang L, Zhang F, Hu R. Experimental oral immunization of ferret badgers (Melogale moschata) with a recombinant canine adenovirus vaccine CAV-2-E3D-RGP and an attenuated rabies virus SRV9. J Wildl Dis. 2014; 50(2): 374-7.

69. Шмаров MM, Тутыхина ИЛ, Логунов ДЮ, Верховская ЛВ, Народицкий БС, Гинцбург АЛ. Индукция протективного иммунного ответа у мышей, вакцинированных рекомбинантным аденовирусом птиц CELO, экспрессирующим гликопротеин G вируса бешенства. Журнал микробиологии, эпидемиологии и иммунобиологии 2006; (4): 69-71.

70. Xiang ZQ, Greenberg L, Ertl HC, Rupprecht CE. Protection of non-human primates against rabies with an adenovirus recombinant vaccine. Virology 2014; 450-451: 243-9.

71. Tutykhina IL, Logunov DY, Shcherbinin DN, Shmarov MM, Tukhvatulin AI, Naroditsky BS, et al. Development of adenoviral vector-based mucosal vaccine against influenza. J Mol Med (Berl). 2011; 89(4): 331-41.

72. Yarosh OK, Wandeler AI, Graham FL, Campbell JB, Prevec L. Human adenovirus type 5 vectors expressing rabies glycoprotein. Vaccine 1996; 14(13): 1257-64.

73. Shen CF, Lanthier S, Jacob D, Montes J, Beath A, Beresford A, et al. Process optimization and scale-up for production of rabies vaccine live adenovirus vector (AdRG1.3). Vaccine 2012; 30(2): 300-6.

74. Lutze-Wallace C, Wandeler A, Prevec L, Sidhu M, Sapp T, Armstrong J. Characterization of a human adenovirus 5: rabies glycoprotein recombinant vaccine reisolated from orally vaccinated skunks. Biologicals 1995; 23(4): 271-7.

75. Knowles MK, Nadin-Davis SA, Sheen M, Rosatte R, Mueller R, Beresford A. Safety studies on an adenovirus recombinant vaccine for rabies (AdRG1.3-ONRAB®) in target and non-target species. Vaccine 2009; 27(47): 6619-26.

76. Knowles MK, Roberts D, Craig S, Sheen M, Nadin-Davis SA, Wandeler AI. In vitro and in vivo genetic stability studies of a human adenovirus type 5 recombinant. Vaccine 2009; 27(20): 2662-8.

77. Rosatte RC, Donovan D, Davies JC, Brown L, Allan M, von Zuben V, et al. High-density baiting with ONRABH rabies vaccine Baits to control arctic-variant rabies in striped skunks in Ontario, Canada. J Wildl Dis. 2011; 47(2): 459-65.

78. Mainguy J, Rees EE, Canac-Marquis P, Bélanger D, Fehlner-Gardiner C, Séguin G, et al. Oral rabies vaccination of raccoons and striped skunks with ONRAB® baits: multiple factors influence field immunogenicity. J Wildl Dis. 2012; 48(4): 979-90.

79. Sobey KG, Walpole AA, Rosatte R, Fehlner-Gardiner C, Donovan D, Bachmann P, et al. An assessment of ONRAB® oral rabies vaccine persistence in free-ranging mammal populations in Ontario, Canada. Vaccine 2013; 31(17): 2207 - 13.

80. Slate D, Chipman RB, Algeo TP, Mills SA, Nelson KM, Croson CK, et al. Safety and immunogenicity of Ontario rabies vaccine bait (ONRAB) in the first us field trial in raccoons (Procyon lotor). J Wildl Dis. 2014; 50(3): 582-95.

81. Fry TL, Vandalen KK, Duncan C, Vercauteren K. The safety of ONRAB® in select non-target wildlife. Vaccine 2013; 31(37): 3839-42.

82. Centers for Disease Control and Prevention (CDC). Human contacts with oral rabies vaccine baits distributed for wildlife rabies management-Ohio, 2012. MMWR Morb Mortal Wkly Rep. 2013; 62(14): 267-9.

83. Gao GP, Yang Y, Wilson JM. Biology of adenovirus vectors with E1 and E4 deletions for liver-directed gene therapy. J Virol. 1996; 70(12): 8934-43.

84. Wang S, Sun C, Zhang S, Zhang X, Liu Y, Wang Y, et al. Glycoprotein from street rabies virus BD06 induces early and robust immune responses when expressed from a nonreplicative adenovirus recombinant. Arch Virol. 2015; 160(9): 2315-23.


Для цитирования:


Седова Е.С., Шмаров М.М. Новые антирабические рекомбинантные вакцины. БИОпрепараты. Профилактика, диагностика, лечение. 2016;16(4):219-228.

For citation:


Sedova E.S., Shmarov M.M. New recombinant rabies vaccines. BIOpreparations. Prevention, Diagnosis, Treatment. 2016;16(4):219-228. (In Russ.)

Просмотров: 48


Creative Commons License
Контент доступен под лицензией Creative Commons Attribution 4.0 License.


ISSN 2221-996X (Print)
ISSN 2619-1156 (Online)