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The kit for identification of antibodies against Lassa and Ebola viruses by indirect immunofluorescence

Abstract

Timely diagnosis of particularly dangerous infections caused by viruses Ebola and Lassa is crucial in organization of anti-epidemic measures in the case of important of the disease on the territory of the Republic of Belarus. The main method of rapid response at revealing hemorrhagic fevers Lassa and Ebola still is the method of indirect immunofluorescence. In this work the elaboration of the kit for detection of antibodies to exciters of extremely dangerous viral infection Lassa and Ebola by indirect immunofluorescence method is described.

About the Authors

L. M. Rustamova
Republican Research & Practical Centre for Epidemiology and Microbiology
Russian Federation
Leading researcher, head of Specialized collection of viruses and bacteria, pathogenic for humans. Candidate of Medical Sciences


S. F. Semenov
Republican Research & Practical Centre for Epidemiology and Microbiology
Russian Federation
Researcher of Laboratory of biosafety with the collection of pathogens


N. L. Bogdanova
Republican Research & Practical Centre for Epidemiology and Microbiology
Russian Federation
Researcher of Laboratory of biosafety with the collection of pathogens


A. S. Vladyko
Republican Research & Practical Centre for Epidemiology and Microbiology
Russian Federation
Head of Laboratory of biotechnology and immunodiagnostics of especially dangerous virus infections. Doctor of Medical Sciences, professor


A. G. Krasko
Republican Research & Practical Centre for Epidemiology and Microbiology
Russian Federation
Head of Laboratory of biosafety with the collection of pathogens. Candidate of Medical Sciences, docent


References

1. WHO statement after the meeting of the Committee of the International Health Regulations Emergency in relation to outbreaks of Ebola in 2014 in West Africa. Available from: http://www.who.int/ mediacentre/news/statements/2014/ebola-20140808/ru/(in Russian).

2. Krasko AG, Rustamova LM, Scheslenok EP, Semizhon PA, Vladyko AS, Gorbunov VA. Diagnosis and prevention of dangerous viral infections in the Republic of Belarus. In: Proceedings of the XII Inter-State scientific-practical conference, Saratov, 25–26 November 2014. Saratov; 2014. P. 123 (in Russian).

3. Frame ID, Verbrugge GP, Gill RG, Pinneo L. The use of Lassa fever convalescent plasma in Nigeria. Trans R Soc Trop Med Hyd. 1984; 78: 319–24.

4. Trofimov NM, Klimashevskaya LM, Erofeeva NI. The influence of some physical and chemical factors on arenaviruses. Voprosy virusologii 1981; (2): 240–2 (in Russian).

5. Ustinova EN, Shestopalov AM, Bakulina LF, Chepurnov AA. Titration of the Ebola virus and Marburg on the plaque under the semi-liquid agar surface. Voprosy virusologii 2003; (1): 43–4 (in Russian).

6. Markin VA. The methodology of collecting pathogens. Voprosy virusologii 2010; (5): 4–9 (in Russian).


Review

For citations:


Rustamova L.M., Semenov S.F., Bogdanova N.L., Vladyko A.S., Krasko A.G. The kit for identification of antibodies against Lassa and Ebola viruses by indirect immunofluorescence. BIOpreparations. Prevention, Diagnosis, Treatment. 2016;16(2):115-119. (In Russ.)

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ISSN 2221-996X (Print)
ISSN 2619-1156 (Online)