Current status of the laboratory diagnosis of anthrax: detection and identification of Bacillus anthracis
Abstract
The present article summarizes the materials from literary sources dedicated to the issues of laboratory diagnosis of anthrax. It shows the priority of the development of new methods for the elaboration of highly sensitive test systems and preparations designed for the detection and identification of anthrax bacteria. It outlines the role of express-diagnostics of infectious diseases, which is very important when investigating bioterrorism cases, outbreaks and sporadic cases in humans and animals. Providing with the analysis of different methods for detection of anthrax, the article at the same time focuses on modern molecular diagnostic technologies. It is shown that the basic parameters of diagnostic test systems are sensitivity, specificity and reproducibility of the results. The article presents data on the development of culture media for isolation and identification of anthrax, as well as the possibility of using anthrax bacteriophage for phage-based indication and identification of bacteria.
About the Authors
L. V. Sayapina
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation
Chief expert of Office of expertise of antibacterial medical immunobiological preparations of Center for examination and control of medical immunobiological preparations. Doctor of Medical Sciences
Russian Federation
Chief expert of Office of expertise of antibacterial medical immunobiological preparations of Center for examination and control of medical immunobiological preparations. Doctor of Medical Sciences
R. N. Lobach
294th Center for Extra-Risk Rescue Operations
Russian Federation
Head of the group of biological protection
Russian Federation
Head of the group of biological protection
V. P. Bondarev
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation
Director of Center for examination and control of medical immunobiological preparations. Doctor of Medical Sciences, professor
Russian Federation
Director of Center for examination and control of medical immunobiological preparations. Doctor of Medical Sciences, professor
N. F. Nikityuk
Scientific Centre for Expert Evaluation of Medicinal Products
Russian Federation
Chief expert of Office of expertise of antibacterial medical immunobiological preparations of Center for examination and control of medical immunobiological preparations. Doctor of Medical Sciences, professor
Russian Federation
Chief expert of Office of expertise of antibacterial medical immunobiological preparations of Center for examination and control of medical immunobiological preparations. Doctor of Medical Sciences, professor
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Review
For citations:
Sayapina L.V., Lobach R.N., Bondarev V.P., Nikityuk N.F. Current status of the laboratory diagnosis of anthrax: detection and identification of Bacillus anthracis. BIOpreparations. Prevention, Diagnosis, Treatment. 2016;16(1):27-34. (In Russ.)
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