biopreparatБИОпрепараты. Профилактика, диагностика, лечениеBiological Products. Prevention, Diagnosis, Treatment2221-996X2619-1156Scientific Centre for Expert Evaluation of Medicinal Productsbiopreparat-9Research ArticleОБЗОРЫREVIEWSПрямые и косвенные методы определения нуклеотидного состава ДНК последовательностей микроорганизмовDirect and indirect methods of determining DNA nucleotide sequences in microorganismsВолковаР. А.VolkovaR. A.volkova@expmed.ruСколотневаЕ. С.SkolotnevaE. S.noemail@neicon.ruЭльбертЕ. В.ElbertE. V.noemail@neicon.ruМыцаЕ. Д.MytsaE. D.noemail@neicon.ruМеркуловВ. А.MerkulovV. A.noemail@neicon.ruБондаревВ. П.BondarevV. P.noemail@neicon.ruБорисевичИ. В.BorisevichI. V.noemail@neicon.ruФедеральное государственное бюджетное учреждение «Научный центр экспертизы средств медицинского применения» Министерства здравоохранения Российской ФедерацииРоссияFederal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» of the Ministry of Health of the Russian FederationRussian FederationМГУ им. М.В. ЛомоносоваРоссияM.V. Lomonosov Moscow State UniversityRussian Federation20152002201802914Copyright © Волкова Р.А., Сколотнева Е.С., Эльберт Е.В., Мыца Е.Д., Меркулов В.А., Бондарев В.П., Борисевич И.В., 20182018Волкова Р.А., Сколотнева Е.С., Эльберт Е.В., Мыца Е.Д., Меркулов В.А., Бондарев В.П., Борисевич И.В.Volkova R.A., Skolotneva E.S., Elbert E.V., Mytsa E.D., Merkulov V.A., Bondarev V.P., Borisevich I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.biopreparations.ru/jour/article/view/9

Типирование бактериальных линий и идентификация микроорганизмов - чрезвычайно важные задачи в области профилактики, диагностики и лечения бактериальных инфекций. За последние два десятилетия бурно развивающиеся молекулярные подходы к типированию бактериальных линий стали необходимым инструментом для решения этой задачи. Информация, которую могут дать нуклеиновые кислоты, является наиболее полной по сравнению с другими характеристиками организма. Получить ее можно как непосредственным определением избранных последовательностей генома, так и с помощью различных техник, позволяющих, косвенно оценить нуклеотидный состав репрезентативного участка генома исследуемого микроорагнизма. Статья посвящена обзору литературных данных по прямым и косвенным методам определения нуклеотидного состава ДНК последовательностей микроорганизмов (типирование по мультилокусным сик-венсам, типирование по множеству отсеквенированных участков, электрофорез в денатурирующей системе, высокочувствительный анализ кривых плавления, ДНК макро(микро)чипы).

Typing of bacterial populations and identification of microorganisms are the overriding priorities in the field of prophylaxis, diagnosis and treatment of bacterial infections. Over the recent two decades rapidly developing approaches to molecular typing of bacterial populations have become an essential tool for the mentioned task. The information encoded in nucleic acids is more complete as compared to other characteristics of an organism. It can be obtained by either direct determination of genome sequences or by using a variety of techniques, that allow to indirectly assess the nucleotide composition in a representative genome locus of a microorganism under study. The article provides an overview of the literature data on direct and indirect methods of determining nucleotide composition of DNA sequences in microorganisms (multiple locus sequence typing, multiple sequeneed sites typing, denaturing gel electrophoresis, high-sensitivity melting curve analysis, DNA macro(micro)arrays).

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The authors declare that there are no conflicts of interest present.