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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">biopreparat</journal-id><journal-title-group><journal-title xml:lang="ru">БИОпрепараты. Профилактика, диагностика, лечение</journal-title><trans-title-group xml:lang="en"><trans-title>Biological Products. Prevention, Diagnosis, Treatment</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2221-996X</issn><issn pub-type="epub">2619-1156</issn><publisher><publisher-name>Scientific Centre for Expert Evaluation of Medicinal Products</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">biopreparat-14</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ СТАТЬИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ORIGINAL ARTICLES</subject></subj-group></article-categories><title-group><article-title>Динамическое светорассеяние - простой и чувствительный метод, позволяющий определять появление иммунных комплексов в биологических жидкостях</article-title><trans-title-group xml:lang="en"><trans-title>Dynamic light scattering - a simple and sensitive method of determination immune complexes in biological liquids</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кораблев</surname><given-names>П. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Korabliov</surname><given-names>P. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ланда</surname><given-names>С. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Landa</surname><given-names>S. B.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Семенова</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Semionova</surname><given-names>E. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Филатов</surname><given-names>М. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Filatov</surname><given-names>M. V.</given-names></name></name-alternatives><email xlink:type="simple">filatov@omrb.pnpi.spb.ru</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Национальный научно-исследовательский институт Центр инновационной медицины</institution><country>Россия</country></aff><aff xml:lang="en"><institution>State Research Institute Centre for Innovative Medicine</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Петербургский институт ядерной физики им. Б.П. Константинова (НИЦ Курчатовский институт)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>B.P. Konstantinov Petersburg Nuclear Physics Institute (NIC Kurchatov Institute)</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Петербургский институт ядерной физики им. Б.П. Константинова (НИЦ Курчатовский институт), Санкт-Петербургский государственный политехнический университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>B.P. Konstantinov Petersburg Nuclear Physics Institute (NIC Kurchatov Institute), Saint Petersburg State Polytechnic University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2015</year></pub-date><pub-date pub-type="epub"><day>20</day><month>02</month><year>2018</year></pub-date><volume>0</volume><issue>2</issue><fpage>53</fpage><lpage>58</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кораблев П.В., Ланда С.Б., Семенова Е.В., Филатов М.В., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Кораблев П.В., Ланда С.Б., Семенова Е.В., Филатов М.В.</copyright-holder><copyright-holder xml:lang="en">Korabliov P.V., Landa S.B., Semionova E.V., Filatov M.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.biopreparations.ru/jour/article/view/14">https://www.biopreparations.ru/jour/article/view/14</self-uri><abstract><p>В работе предложен новый подход анализа иммунных комплексов, образующихся в гетерогенных биологических жидкостях. Метод основан на анализе вклада иммунных комплексов в динамическое светорассеяние, регистрируемое с помощью лазерного коррелометра с гетеродинной схемой измерения. На модели сыворотки эмбрионов телят, к которой добавлены моноклональные антитела к церулоплазмину, продемонстрировано, что предлагаемый подход способен регистрировать иммунные комплексы, образующиеся при добавлении антигена в концентрации, превышающей 150 пг в мл. С помощью иммуноаффинных методов подтверждена правомерность идентификации пиков на гистограммах динамического светорассеяния в качестве соответствующих иммунным комплексам. Для этих целей было использовано избирательное удаление иммунных комплексов с помощью стафилококкового белка А, иммобилизованного на частицах сефарозы или увеличение размера иммунных комплексов за счет их дальнейшей агрегации с помощью антител, специфичных для иммуноглобулинов человека. Предлагаемый подход позволяет выявлять иммунные комплексы в различных биологических жидкостях.</p></abstract><trans-abstract xml:lang="en"><p>In this paper we introduce new approach in formed in heterogeneous biological fluids immune complexes analysis. The approach is based on an analysis of the contribution of immune complexes in dynamic light scattering, recorded by laser correlometer with heterodyne measurement circuit. We demonstrated our approach sensitivity using embryonic calf serum model in addition of monoclonal antibody against hepatocuprein. Proposed approach is capable to detect forming immune complexes by adding antigen at a concentration greater than 150 pg/ml. Using immunoaffinity techniques we confirmed the lawfulness of identified peaks in the histograms of dynamic light scattering as appropriate immune complexes. For these purposes, we selectively removed immune complexes using staphylococcal protein A sepharose immobilized on the particles or increased in size immune complexes due to their further aggregation using antibodies specific for human immunoglobulin. The proposed approach can detect immune complexes in various biological fluids.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>immune complexes</kwd><kwd>blood plasma</kwd><kwd>diagnostic method</kwd><kwd>dynamic light scattering</kwd><kwd>иммунные комплексы</kwd><kwd>плазма крови</kwd><kwd>диагностический метод</kwd><kwd>динамическое светорассеяние</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Mibei E.K., Orago A.S., Stoute J.A. Immune complex levels in children with severe Plasmodium falciparum malaria. 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